Abstract :Abstract
The present study was design to evaluate the protective effects of ferulic acid and ascorbic acid on aniline induced spleen toxicity in rats. Wistar rats of either sex were used in the study. Spleen toxicity was induced by aniline hydrochloride (100 ppm) in drinking water for 30 days. Treatment group received ferulic acid (40 mg/kg/day, p.o) and ascorbic acid (40 mg/kg/day, p.o) alone and in combination for 30 days. At the end of treatment period serum and tissue parameters were evaluated. Aniline hydrochloride treated rats showed a significant alteration in body weight, spleen weight, feed consumption and water intake, haematological parameters (Haemoglobin, Red Blood Cells, White Blood Cells and Total Iron Content), biochemical parameters (total iron content, total protein, Lipid peroxidation, Reduced glutathione, nitric oxide) and Membrane bound phosphatase (ATPase). Treatment with combination of ferulic acid and ascorbic acid 40 mg/kg respectively showed a significant recovery in aniline induce spleen toxicity. Combination of ferulic acid and ascorbic acid showed better effects than alone antioxidants in aniline hydrochloride induced spleen toxicity.